GC-MS 6800 to detect acrylamide solutions in foods - Database & Sql Blog Articles

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Acrylamide is a polar small molecule compound that has raised significant concerns due to its potential neurotoxicity, genotoxicity, and carcinogenicity. The presence of acrylamide in food has drawn the attention of international organizations and governments. In 2002, the Swedish National Food Authority first reported high levels of acrylamide in certain fried or baked foods, marking a turning point in awareness about this contaminant.

Acrylamide primarily forms during the cooking of high-carbohydrate, low-protein vegetable foods at temperatures above 120°C. A 2012 study by the British Food Standards Agency tested 248 food samples and found that 13 types of food contained increased levels of acrylamide, which is considered a known carcinogen. In response, China’s Ministry of Health issued a report in 2005 assessing the health risks of acrylamide in food, recommending reduced consumption of fried products to minimize health risks.

The complexity of food composition and the low concentration of acrylamide make its detection challenging. In China, two main methods are used for detecting acrylamide in food: GB/T 5009.204-2014 and SN/T 2096-2008. While SN/T 2008 aligns with methods used by the U.S. FDA, the use of internal standard-labeled acrylamide is expensive and hard to obtain, limiting the practical application of these techniques.

Tianrui Instruments offers an advanced solution for acrylamide detection through its GC-MS 6800 Gas Chromatography-Mass Spectrometer. This system is designed based on the GB/T 5009.204-2014 method and provides a complete, reliable solution for food safety testing. It ensures accurate and consistent results, making it ideal for laboratories focused on food contamination analysis.

The GC-MS 6800 is a cost-effective gas chromatography-mass spectrometer developed by Tianrui Instruments. It features independent intellectual property rights and several patented technologies, making it suitable for a wide range of applications including environmental monitoring, pharmaceuticals, petrochemicals, and food safety testing. Its versatility and reliability have made it a popular choice among researchers and laboratories worldwide.

Figure 1. Appearance of GC-MS 6800 Gas Chromatography Mass Spectrometer

Acrylamide is an unsaturated amide, also known as AM. It appears as a colorless transparent flake crystal with a boiling point of 125°C (3325 Pa) and a melting point of 84–85°C. Its density is 1.122 g/cm³. It is soluble in water, ethanol, acetone, and chloroform but insoluble in benzene and heptane. Acrylamide can hydrolyze into acrylic acid under acidic or basic conditions.

GC-MS 6800 (Jiangsu Tianrui Instrument Co., Ltd.), rotary evaporator, shaking device, centrifuge (≤10,000 rpm), vortex mixer, nitrogen evaporation instrument, hexane, ethyl acetate, anhydrous sodium sulfate, ammonium sulfate, sodium thiosulfate, bromine, hydrobromic acid, potassium bromide, ultrapure water (conductivity ≤0.01 mS/m at 25°C), and a diatomite column (SLE-SPE 20g/60mL).

Acrylamide was extracted from food samples using ultrapure water at 60°C, then purified using a diatomite column and derivatized with bromine reagent. The sample was analyzed using gas chromatography-mass spectrometry. Identification was based on retention time and characteristic mass spectral ions, with external standard calibration for quantification. The bromination reaction is represented as: CH₂=CHCONH₂ + Br₂ → CH₂BrCHBrCONH₂.

Column: DB-5MS, 30m × 0.25mm × 0.25μm; Oven temperature program: 120°C (3 min) ramped at 30°C/min to 240°C (10 min); Injection port temperature: 240°C; Interface temperature: 240°C; Ion source temperature: 240°C; Carrier gas: helium (≥99.999%), flow rate 1.0 mL/min; Ionization mode: EI; Ionization energy: 70 eV; Injection method: splitless, injection volume: 1.0 μL; Detection method: TIC (full scan) for qualitative analysis and SIM mode for quantitative analysis.

Figure 2. Total Ion Chromatogram of Acrylamide Derivative Under Full Scan

Figure 3. Mass Spectrum of Acrylamide Derivatives

The GC-MS 6800 is a powerful tool for detecting acrylamide in food. It delivers precise and reliable results, making it a valuable asset for laboratories involved in food safety testing. With its advanced technology and user-friendly design, it helps ensure compliance with regulatory standards and supports the development of safer food products.