DNA and RNA extraction methods for fungi - Database & Sql Blog Articles

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Fungal DNA and RNA Extraction Methods When studying the function of fungal genes, DNA and RNA extraction is always an essential step. However, due to the high content of polysaccharides in fungi, it can be challenging to obtain high-quality nucleic acids that are suitable for downstream analysis. Therefore, finding a cost-effective and reliable method is crucial. In this article, I will share a method I have been using successfully for fungal DNA and RNA extraction. It's a practical and efficient approach that can be applied in most laboratory settings. **Fungal DNA Extraction Method (Method 1)** 1. Take 0.5g of fungal mycelium and quickly grind it into a fine powder in liquid nitrogen to prevent degradation. 2. Add 4mL of extraction buffer and mix by shaking vigorously. 3. Add an equal volume of isoamyl alcohol (in a 24:1 ratio with the extraction buffer) and vortex for 3â€“5 minutes. This step helps reduce contamination without the need for phenol, saving both time and cost. 4. Centrifuge at 1000 rpm, 4Â°C for 5 minutes. 5. Transfer the supernatant to a new tube and re-extract with isoamyl alcohol (24:1). Centrifuge again at 10,000 rpm, 4Â°C for 5 minutes. 6. Collect the supernatant, add 2/3 volume of pre-cooled isopropanol (-20Â°C) or 2.5 volumes of absolute ethanol, mix well, and let stand for about 30 minutes. 7. Use a capillary rod to collect the precipitate, rinse it several times with 75% ethanol, once with absolute ethanol, air dry, and resuspend in 500 ÂµL of TE buffer. 8. Add 1 ÂµL of RNase A (10 mg/mL) and incubate at 37Â°C for 1 hour to remove any residual RNA. 9. Perform a final phenol (pH 8.0): isoamyl alcohol (25:24:1) extraction. Centrifuge at 10,000 rpm, 4Â°C for 5 minutes. 10. Take the supernatant, add 1/10 volume of 3M NaAc and 2.5 volumes of absolute ethanol, then incubate at -70Â°C for over 30 minutes. 11. Rinse the precipitate with 75% ethanol, air dry, and dissolve in 200 ÂµL of TE buffer. Store at -20Â°C for long-term use. **RNA Extraction Tips** For RNA extraction, you can modify the above method slightly by avoiding the addition of RNase A and instead using a dedicated RNA extraction kit or adding a small amount of glycogen as a co-precipitant to improve yield. Also, ensure all glassware and tools are RNase-free to avoid contamination. These methods are designed to be simple, effective, and adaptable for various fungal species. Whether you're working on basic research or industrial applications, having a solid extraction protocol is key to obtaining accurate and reproducible results. Let me know if you'd like further details or alternative protocols!
Photocell Timer

24HR Electronic Timer socket with photocell.

â‘ Light intensity setting

â‘¡ Light intensity detection

â‘¢ Countdown Timer ON & OFF

â‘£ 4 MODES:

Set > Detected: When the light intensity detection value is less than the set value, switch ON or OFF.

Detected > Set: When the light intensity detection value is greater than the set value, switch ON or OFF

ON : Always ON

OFF : Always OFF

NOTED:

1. The light intensity displayed by this machine is not the standard light intensity value (Lux), only the relative light intensity value.

2. The light intensity value is affected by the placement position and direction. Please determine the position first and then set it according to the actual light intensity detected. If you change the position or change the orientation, you need to reset the light intensity setting value suitable for the new position.

3. This product has built-in rechargeable battery. If it is not connected to AC for a long time, you need to connect the power supply to charge until the LCD can display normally.

MANUAL OPERATION

1. Press [UP" or [DOWN" to set the LUX value.

2. Press the [SET" key to start setting, and the P1 settable items will be flashed.

3. Press [UP" or [DOWN" to adjust the value.

4. Press [SET" key again to exit setting or enter next setting for countdown timer.

5. Repeat the [SET" key to start setting, and the P2 & P3 settable items will be flashed.

6. Press the [FUN" key to switch the working state in the following:

Set > Detected -> Detected > Set -> ON -> OFF

Set > Detected: Automatically switches when the detected ambient light intensity is darker than the set value

Detected >Set: Automatically switch when the detected ambient light intensity is brighter than the set value

When the brightness meets the setting conditions, the countdown starts as below:

Note:when the countdown is ON, the detected value is not displayed.

When the brightness does not meet the setting conditions, the countdown stops and waits:

After the countdown ON is reduced to 0, the countdown OFF starts immediately and the power is OFF.

After the countdown OFF is reduced to 0:

A. If the light intensity meets the set conditions, a new round of countdown will be started;

B. If the light intensity does not meet the set conditions, keep the power off and wait for the light to meet the conditions before turning on automatically.

NOTE:

1. If the power is cut off while the countdown is running, the countdown will be terminated immediately and the relay output will be off. After the power is turned on again, a new round of brightness detection will start.

2. Modifying the brightness value in the countdown operation will not affect the current countdown operation. After the off time of the current countdown, the new brightness setting value will take effect.

3. In the countdown on operation, change the setting value of the countdown on, this countdown will still be timed according to the original setting value; the new setting value will take effect when the next countdown on starts.

4. In the countdown off operation, change the setting value of countdown off, this countdown will still be timed according to the original setting value; the new setting value will take effect when the next countdown off is started.

NOTE: the brightness setting value, countdown ON or countdown OFF, any one of which is equal to 0, cannot be switched ON or OFF automatically.

Manual Control

When ON or OFF is displayed, it means that the power supply remains ON or OFF, as shown in the figure below:

Power Detection and Standby Mode

With AC power supply, the icon lights up and works normally.

When there is no AC power supply, the icon goes out, the brightness is not detected at this time, and the system enters the standby mode.

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NINGBO COWELL ELECTRONICS & TECHNOLOGY CO., LTD , https://www.cowellsocket.com