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Customers onlookers "buy how to save antibodies" - Database & Sql Blog Articles
**How to Properly Store Antibodies for Long-Term Use**
As the year comes to an end, many of our customers reach out asking about how to best preserve the antibodies they’ve purchased. With the holiday season approaching, it’s common to have a long break from the lab, and you might be wondering: Can I still use my antibodies next year? How should I store them to maintain their effectiveness?
At Shanghai Jinma Bio, we’ve been selling Abcam and other high-quality antibody brands for years, and we’ve gained extensive experience in handling and preserving these critical reagents. Let’s walk through the best practices for storing and transporting your antibodies to ensure their longevity and performance.
Once you receive your antibody, always centrifuge it at 4°C, 12,000 rpm for 3 minutes (whether it's liquid or lyophilized). This step ensures that all the antibody material is collected at the bottom of the tube, minimizing any loss during dispensing. If the volume is less than 50 µL, extend the centrifugation time to 5 minutes. It’s important to note that even if not fully centrifuged at lower speeds, this step helps prevent incomplete recovery of the antibody.
For most ProSci antibodies, the recommended storage conditions are -20°C or -80°C after aliquoting. Here are some key tips:
- **Storage temperature**: For most antibodies, -20°C is sufficient. There’s no strong evidence that -80°C offers better preservation.
- **Aliquoting**: Always divide the antibody into single-use portions to avoid repeated freeze-thaw cycles. Each aliquot should be at least 10 µL to minimize evaporation and wall adsorption.
- **Reconstituted antibodies**: If you reconstitute the antibody but don’t use it immediately, store the remaining solution at 4°C, not in the freezer.
- **Working solutions**: Use the diluted antibody on the same day. If stored at 4°C, do not keep it for more than 24 hours.
- **Avoid automatic defrost refrigerators**: These can cause temperature fluctuations, leading to unnecessary freeze-thaw events. Always store antibodies in the inner part of the fridge.
Most antibodies can be stored at 4°C for 1–2 weeks without affecting their activity. During shipping, antibodies are typically transported at 4°C for 1–2 weeks to prevent damage from repeated freezing and thawing. Dry ice should be avoided, as it causes the antibody to freeze upon arrival, requiring additional thawing steps that can harm its structure.
Regarding sodium azide: Many antibodies contain 0.02%–0.05% sodium azide to prevent microbial contamination. If you need to remove it, you can do so via dialysis or filtration using a membrane with a 14kDa cutoff. Sodium azide has a low molecular weight (65 Da), while IgG is around 150 kDa, making it easy to separate.
If you're looking for special offers on antibodies this season, visit our website or contact our customer service for more details. Thank you for choosing Shanghai Jinma Bio — we appreciate your trust and wish you a great shopping experience!