July 17, 2025

Baicalin sample analysis report - Database & Sql Blog Articles

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Batch Number: R08D5F1

Sample NameBaicalin
CAS No.21967-41-9
Molecular FormulaC21H18O11
Molecular Weight446.37
Structure
Storage Conditions4°C, sealed, protected from light
ProjectStandardResult
ExteriorLight yellow powderMeets the standard
SolubilityAlkaline aqueous solutionMeets the standard
HPLC98%98.1%

Material: Hyper 0DS2 C18

Flow Rate: 1.0 ml/min

Mobile Phase: Methanol : 0.4% Phosphate water = 55:45

Pressure: 10.0 MPa

Column Length: 250 mm

Detector: DAD at 270 nm

Column Diameter: 4.6 mm

Injection Volume: 20 µL

#

Component Name

Retention Time (min)

Peak Height (mV)

Peak Area (mV·s)

Area Percentage (%)

1 Unknown 11.62167 5.46 53.05 0.5422

2 Unknown 13.41833 2.02 12.28 0.1255

3 Baicalin 13.71667 997.61 9604.04 98.1526

4 Unknown 15.56833 10.24 91.04 0.9304

5 Unknown 19.36333 3.34 24.39 0.2493

This HPLC analysis was conducted to determine the purity and composition of the Baicalin sample with batch number R08D5F1. The results show that the sample has a high purity level of 98.1%, which is very close to the specified standard of 98%. The chromatogram indicates that the main component, Baicalin, elutes at a retention time of approximately 13.72 minutes, with a peak area percentage of 98.15%, confirming its dominance in the sample. Several unknown impurities were also detected, but their contribution to the total area is minimal. The analysis was performed using a reversed-phase column (Hyper 0DS2 C18) under optimized conditions, including a mobile phase of methanol and 0.4% phosphate buffer at a ratio of 55:45, with a flow rate of 1.0 mL/min and detection at 270 nm. These parameters ensure accurate and reproducible results for the quantification of Baicalin in this sample.

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